7BRC

Crystal structure of the TMK3 LRR domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2020-01-12
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979183
Spacegroup name	P 1 21 1
Unit cell lengths	49.340, 115.610, 57.860
Unit cell angles	90.00, 92.35, 90.00

Refinement procedure

Resolution	38.290 - 2.060
R-factor	0.1783
Rwork	0.177
R-free	0.20580
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4hq1
RMSD bond length	0.009
RMSD bond angle	0.932
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	57.810	2.110
High resolution limit [Å]	2.060	2.060
Number of reflections	39810	2890
<I/σ(I)>	10.6
Completeness [%]	99.4
Redundancy	6.6
CC(1/2)	0.994	0.666

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	4.6	289	0.2 M ammonium sulfate, 0.1M sodium acetate trihydrate pH 4.6, 30% W/V PEG monomethyl ether 2000, 0.05 M sodium fluoride