7BQZ
Crystal Structure of Spindlin1 bound to H3(K4me3-K9me3) peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-04-09 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.521, 142.681, 132.295 |
| Unit cell angles | 90.00, 95.39, 90.00 |
Refinement procedure
| Resolution | 48.390 - 3.101 |
| R-factor | 0.226 |
| Rwork | 0.224 |
| R-free | 0.26390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ns2 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.885 |
| Data reduction software | DENZO |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.150 |
| High resolution limit [Å] | 3.100 | 8.400 | 3.100 |
| Rmerge | 0.130 | 0.054 | 0.647 |
| Rmeas | 0.154 | 0.065 | 0.759 |
| Rpim | 0.082 | 0.036 | 0.395 |
| Total number of observations | 92299 | ||
| Number of reflections | 26618 | 1372 | 1331 |
| <I/σ(I)> | 6.2 | ||
| Completeness [%] | 98.5 | 98.5 | 99.2 |
| Redundancy | 3.5 | 3.3 | 3.6 |
| CC(1/2) | 0.996 | 0.795 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 291 | 2.8M sodium acetate, pH7.0 |
