7BQI
Crystal structure of FYCO1 RUN domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NE3A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-11-15 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 48.119, 48.119, 142.671 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.600 - 1.300 |
| R-factor | 0.2129 |
| Rwork | 0.211 |
| R-free | 0.23990 |
| Structure solution method | SIRAS |
| Starting model (for MR) | SAD model |
| RMSD bond length | 0.030 |
| RMSD bond angle | 2.434 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AutoSol |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.320 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.092 | 0.540 |
| Number of reflections | 38506 | 1885 |
| <I/σ(I)> | 41.6 | 4.5 |
| Completeness [%] | 90.7 | |
| Redundancy | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 20% (w/v) PEG 3350, 180mM tri-ammonium citrate |
