7BQ5
ZIKV sE bound to mAb Z6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-03-27 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 158.245, 153.672, 115.201 |
| Unit cell angles | 90.00, 125.49, 90.00 |
Refinement procedure
| Resolution | 39.230 - 2.990 |
| R-factor | 0.2617 |
| Rwork | 0.261 |
| R-free | 0.27540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5jhl |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.733 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.110 |
| High resolution limit [Å] | 2.990 | 3.000 |
| Rpim | 0.094 | |
| Number of reflections | 44965 | 4462 |
| <I/σ(I)> | 7.8 | |
| Completeness [%] | 99.8 | |
| Redundancy | 11.7 | |
| CC(1/2) | 0.555 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1 M Amino Acids, 0.1 M Buffer system 3 at pH 8.5 and 30% v/v Precipitant Mix 3 |
