7BPV
r(GUGGGCCGAC)/d(GTCGGCCCAC) hybrid duplex structure
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE TPS 05A |
Synchrotron site | NSRRC |
Beamline | TPS 05A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2020-02-19 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 0.99984 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 28.348, 46.647, 47.859 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 21.614 - 1.781 |
R-factor | 0.22 |
Rwork | 0.218 |
R-free | 0.23780 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6l75 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 1.840 |
High resolution limit [Å] | 1.780 | 3.830 | 1.780 |
Rmerge | 0.048 | 0.037 | 0.452 |
Rmeas | 0.053 | 0.041 | 0.496 |
Rpim | 0.021 | 0.017 | 0.199 |
Total number of observations | 40863 | ||
Number of reflections | 6395 | 675 | 601 |
<I/σ(I)> | 16.4 | ||
Completeness [%] | 98.8 | 93.1 | 98 |
Redundancy | 6.4 | 5.7 | 5.7 |
CC(1/2) | 0.998 | 0.913 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 5 mM MgSO4, 50 mM Tris HCL, 50 Mm MES (pH-6.5), 2.9 M 1,6-Hexandiol |