7BPS
Crystal structure of mouse TEX101
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2017-11-19 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 138.600, 211.545, 37.286 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.080 - 2.350 |
| R-factor | 0.2015 |
| Rwork | 0.199 |
| R-free | 0.25030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | D_1300016273 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.774 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 45.140 | 45.140 | 2.430 |
| High resolution limit [Å] | 2.350 | 9.100 | 2.350 |
| Rmerge | 0.093 | 0.024 | 0.875 |
| Rmeas | 0.100 | 0.027 | 0.948 |
| Rpim | 0.037 | 0.010 | 0.359 |
| Total number of observations | 169680 | 2820 | 15445 |
| Number of reflections | 23547 | 475 | 2248 |
| <I/σ(I)> | 18 | 58.7 | 2.3 |
| Completeness [%] | 100.0 | 98.9 | 100 |
| Redundancy | 7.2 | 5.9 | 6.9 |
| CC(1/2) | 0.999 | 1.000 | 0.831 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 25% (w/v) PEG4000 0.1 M MES pH 5.5 0.15 M ammonium sulfate |
