7BPG
Structure of serinol nucleic acid - RNA complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 95 |
Detector technology | PIXEL |
Collection date | 2019-10-18 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.919744 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 40.363, 46.918, 47.540 |
Unit cell angles | 90.00, 114.54, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.700 |
R-factor | 0.234 |
Rwork | 0.232 |
R-free | 0.27100 |
Structure solution method | MAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.568 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | AutoSol |
Refinement software | REFMAC (5.8.0266) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.920 | 1.730 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.052 | 0.970 |
Rmeas | 0.062 | 1.144 |
Rpim | 0.032 | 0.600 |
Number of reflections | 17920 | 917 |
<I/σ(I)> | 14.3 | 2 |
Completeness [%] | 99.8 | 98.5 |
Redundancy | 3.5 | |
CC(1/2) | 0.998 | 0.734 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 25% PEG 400, 0.1M HEPES (pH6.5), 75mM Calcium chloride |