7BPB
Human AAA+ ATPase VCP mutant - T76E, AMP-PNP bound form, Conformation I
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | FEI FALCON III (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 4.3 |
| Software | cisTEM,EPU,RELION,PHENIX |
| Symmetry | C6 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
