7BP2
Structural mechanism directing nucleosome reorganization by NAP1-RELATED PROTEIN 1 (NRP1)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-01-12 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97853 |
Spacegroup name | P 31 |
Unit cell lengths | 65.712, 65.712, 96.687 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 28.450 - 1.580 |
R-factor | 0.1564 |
Rwork | 0.155 |
R-free | 0.18630 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1aoi |
RMSD bond length | 0.018 |
RMSD bond angle | 1.295 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | REFMAC |
Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 1.640 |
High resolution limit [Å] | 1.580 | 3.400 | 1.580 |
Rmerge | 0.049 | 0.037 | 0.299 |
Rmeas | 0.052 | 0.039 | 0.321 |
Rpim | 0.016 | 0.012 | 0.116 |
Number of reflections | 64079 | 6436 | 6348 |
<I/σ(I)> | 12.3 | ||
Completeness [%] | 99.9 | 100 | 99.9 |
Redundancy | 8.7 | 10.2 | 7.1 |
CC(1/2) | 0.999 | 0.932 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 0.2M Sodium sulphate 0.1M Bis Tris propane pH 7.5 20% w/v PEG 3350 |