7BOR
Structure of Pseudomonas aeruginosa CoA-bound OdaA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-11-20 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97852 |
| Spacegroup name | P 3 |
| Unit cell lengths | 81.546, 81.546, 59.174 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 26.690 - 1.901 |
| R-factor | 0.1914 |
| Rwork | 0.189 |
| R-free | 0.22590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4nek |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.429 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.320 | 1.969 |
| High resolution limit [Å] | 1.900 | 1.901 |
| Rmeas | 0.073 | 0.677 |
| Number of reflections | 34519 | 3452 |
| <I/σ(I)> | 17.96 | 1.47 |
| Completeness [%] | 99.6 | |
| Redundancy | 4.6 | |
| CC(1/2) | 0.958 | 0.915 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 0.1M HEPES pH7.5, 20% Tacsimate, 2% PEG200 |
