7BOM
Crystal structure of recombinant horse spleen apo-R52C/E56C/R59C/E63C-Fr immobilized with gold ions.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-11-10 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.03556 |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 181.716, 181.716, 181.716 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.730 - 1.930 |
| R-factor | 0.16475 |
| Rwork | 0.163 |
| R-free | 0.19614 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dat |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.724 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.730 | 2.000 |
| High resolution limit [Å] | 1.930 | 1.930 |
| Rmeas | 0.061 | 0.319 |
| Rpim | 0.018 | 0.094 |
| Number of reflections | 19871 | 1952 |
| <I/σ(I)> | 12.76 | |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 11.6 | |
| CC(1/2) | 0.980 | 0.980 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | Ammonium sulphate, Cadmium sulphate |
