7BNY

Structure of 2A protein from encephalomyocarditis virus (EMCV)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-24
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97958
Spacegroup name	P 62 2 2
Unit cell lengths	91.560, 91.560, 316.390
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.910 - 2.620
R-factor	0.2267
Rwork	0.225
R-free	0.25110
Structure solution method	MAD
RMSD bond length	0.003
RMSD bond angle	0.741
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	SHARP
Refinement software	PHENIX (1.18.1_3865)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.910	2.670
High resolution limit [Å]	2.620	2.620
Rmerge	0.227	2.766
Rpim	0.053	0.640
Number of reflections	24668	1179
<I/σ(I)>	11.5	1
Completeness [%]	100.0	99.8
Redundancy	18.9	19.4
CC(1/2)	0.998	0.728

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.7	294	Purified EMCV 2A was concentrated to 5.9 mg/ml in 10 mM HEPES pH 7.9, 1.0 M NaCl, 2.0 mM DTT Drops were prepared by mixing 200 nL protein and 200 nL crystallization buffer: 0.625 M ammonium sulfate, 0.15 M tri-sodium citrate pH 5.7
1	VAPOR DIFFUSION, SITTING DROP	5.7	294	Purified EMCV 2A was concentrated to 5.9 mg/ml in 10 mM HEPES pH 7.9, 1.0 M NaCl, 2.0 mM DTT Drops were prepared by mixing 200 nL protein and 200 nL crystallization buffer: 0.625 M ammonium sulfate, 0.15 M tri-sodium citrate pH 5.7