7BNR
Crystal structure of a ParB Q52A mutant from Myxococcus xanthus bound to CTPyS
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P14 (MX2) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-11-20 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97626 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 45.750, 80.870, 135.710 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.350 - 1.700 |
R-factor | 0.1837 |
Rwork | 0.182 |
R-free | 0.21070 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7bnk |
RMSD bond length | 0.007 |
RMSD bond angle | 1.253 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.390 | 1.550 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.060 | |
Number of reflections | 56287 | 7929 |
<I/σ(I)> | 19.67 | 0.74 |
Completeness [%] | 99.8 | |
Redundancy | 12.8 | |
CC(1/2) | 1.000 | 0.457 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M Tris pH 8.5, 20% PEG 1000 |