7BNP

Llama nanobody nb17 raised against GldL from Flavobacterium jonhsoniae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-01-09
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.978
Spacegroup name	C 1 2 1
Unit cell lengths	42.706, 45.471, 58.100
Unit cell angles	90.00, 108.88, 90.00

Refinement procedure

Resolution	30.200 - 1.700
R-factor	0.2141
Rwork	0.213
R-free	0.22610
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1kxq
RMSD bond length	0.009
RMSD bond angle	0.950
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	BUSTER (2.10.4 (11-DEC-2020))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.200	1.790
High resolution limit [Å]	1.670	1.670
Number of reflections	11641	6051
<I/σ(I)>	15.2
Completeness [%]	95.0
Redundancy	3.1
CC(1/2)	0.999	0.640

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	100mM Tris pH 8.5, 25% PEG 3350