7BNP
Llama nanobody nb17 raised against GldL from Flavobacterium jonhsoniae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-01-09 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.978 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 42.706, 45.471, 58.100 |
Unit cell angles | 90.00, 108.88, 90.00 |
Refinement procedure
Resolution | 30.200 - 1.700 |
R-factor | 0.2141 |
Rwork | 0.213 |
R-free | 0.22610 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1kxq |
RMSD bond length | 0.009 |
RMSD bond angle | 0.950 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | BUSTER (2.10.4 (11-DEC-2020)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.200 | 1.790 |
High resolution limit [Å] | 1.670 | 1.670 |
Number of reflections | 11641 | 6051 |
<I/σ(I)> | 15.2 | |
Completeness [%] | 95.0 | |
Redundancy | 3.1 | |
CC(1/2) | 0.999 | 0.640 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 100mM Tris pH 8.5, 25% PEG 3350 |