7BNK

Crystal structure of ParB from Myxococcus xanthus bound to CDP and Monothiophosphate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-06-04
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9184
Spacegroup name	P 21 21 21
Unit cell lengths	44.243, 80.706, 143.380
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.280 - 1.900
R-factor	0.2051
Rwork	0.203
R-free	0.23660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6sdk
RMSD bond length	0.005
RMSD bond angle	0.842
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.280	1.968
High resolution limit [Å]	1.900	1.900
Rmerge	0.196	2.221
Number of reflections	41375	4071
<I/σ(I)>	9.21	0.83
Completeness [%]	99.9
Redundancy	7.3
CC(1/2)	0.996	0.457

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	10% (v/v) Glycerol 0.2M MgCl2, 0.1M Tris pH 8.5, 25 % (v/v) 1,2-Propanediol