7BMY

p62PH in cesium chloride (0.7 M CsCl in crystallization condition and cryo protectant)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-11-24
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.77114
Spacegroup name	C 2 2 21
Unit cell lengths	34.426, 106.791, 75.870
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.670 - 1.800
R-factor	0.1847
Rwork	0.183
R-free	0.21450
Structure solution method	SAD
RMSD bond length	0.020
RMSD bond angle	2.108
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	43.670	43.670	1.840
High resolution limit [Å]	1.800	9.000	1.800
Rmerge	0.150	0.105	1.359
Rmeas	0.152	0.107	1.392
Rpim	0.026	0.018	0.293
Total number of observations		4358	10544
Number of reflections	12670	137	487
<I/σ(I)>	22	50.8	2.7
Completeness [%]	94.9	99.5	65.1
Redundancy	33	31.8	21.7
CC(1/2)	0.999	0.999	0.851

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	0.7 M cesium chloride, 18 % (w/v) PEG 4000