7BMX
p62PH in cesium chloride (0.25 M CsCl in protein buffer and 0.75 M CsCl in cryo protectant)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-10-30 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.7712 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 34.274, 106.783, 75.319 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.560 - 1.900 |
R-factor | 0.1858 |
Rwork | 0.184 |
R-free | 0.21640 |
Structure solution method | SAD |
RMSD bond length | 0.019 |
RMSD bond angle | 1.940 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | SHELXDE |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 43.560 | 43.560 | 1.940 |
High resolution limit [Å] | 1.900 | 9.110 | 1.900 |
Rmerge | 0.071 | 0.067 | 0.266 |
Rmeas | 0.074 | 0.070 | 0.278 |
Rpim | 0.021 | 0.021 | 0.080 |
Total number of observations | 1421 | 7410 | |
Number of reflections | 10885 | 129 | 658 |
<I/σ(I)> | 25.5 | 45.2 | 9.2 |
Completeness [%] | 96.3 | 99.5 | 92.5 |
Redundancy | 11.5 | 11 | 11.3 |
CC(1/2) | 0.999 | 0.997 | 0.983 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 0.9 M potassium chloride, 17 % (w/v) PEG 4000 |