7BMO
HEWL in cesium chloride (0.25 M CsCl in protein buffer)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2) |
| Synchrotron site | PETRA III, EMBL c/o DESY |
| Beamline | P14 (MX2) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-12-01 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.7712 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 78.628, 78.628, 36.755 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.310 - 1.900 |
| R-factor | 0.2105 |
| Rwork | 0.208 |
| R-free | 0.25430 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.572 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 39.310 | 39.310 | 1.940 |
| High resolution limit [Å] | 1.900 | 9.110 | 1.900 |
| Rmerge | 0.061 | 0.030 | 1.650 |
| Rmeas | 0.062 | 0.031 | 1.714 |
| Rpim | 0.013 | 0.007 | 0.454 |
| Total number of observations | 2176 | 8017 | |
| Number of reflections | 9509 | 116 | 582 |
| <I/σ(I)> | 35.8 | 83.9 | 1.5 |
| Completeness [%] | 99.5 | 99.5 | 96.1 |
| Redundancy | 19.7 | 18.8 | 13.8 |
| CC(1/2) | 1.000 | 1.000 | 0.684 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293.15 | 50 mM sodium acetate pH 4.5, 1.71 M sodium chloride |
