7BMF
Crystal structure of RecJCdc45 from Methanothermobacter thermoautotroficus in complex with dCTP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-11-26 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 |
| Unit cell lengths | 53.750, 53.880, 86.720 |
| Unit cell angles | 79.86, 82.77, 63.98 |
Refinement procedure
| Resolution | 42.210 - 2.200 |
| R-factor | 0.2558 |
| Rwork | 0.254 |
| R-free | 0.29910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6tvv |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.805 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.230 | 2.270 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 42571 | 3602 |
| <I/σ(I)> | 3.4 | 2 |
| Completeness [%] | 97.6 | |
| Redundancy | 2 | |
| CC(1/2) | 0.942 | 0.831 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1M bis-Tris propane pH 6.5, 14% w/v PEG 3350, 0.2M sodium nitrate, 1mM MnCl2 |
