7BM6
Structure-function analysis of a new PL17 oligoalginate lyase from the marine bacterium Zobellia galactanivorans DsijT
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 2 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-01-25 |
Detector | DECTRIS EIGER2 X 9M |
Wavelength(s) | 0.98 |
Spacegroup name | P 65 |
Unit cell lengths | 164.132, 164.132, 168.370 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 45.000 - 2.160 |
R-factor | 0.1547 |
Rwork | 0.153 |
R-free | 0.18220 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7bjt |
RMSD bond length | 0.016 |
RMSD bond angle | 1.688 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.000 | 2.210 |
High resolution limit [Å] | 2.160 | 2.160 |
Rmerge | 1.570 | |
Rpim | 0.054 | 0.356 |
Number of reflections | 137171 | 7343 |
<I/σ(I)> | 16.3 | |
Completeness [%] | 99.6 | 99.2 |
Redundancy | 21.16 | 18 |
CC(1/2) | 0.999 | 0.641 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 296 | protein solution at 13.4 mg.ml-1, supplemented with 1.6 mM of oligo-mannuronate of DP3, was mixed with 0.2 micro-l of crystallization solution that contained 2.4 M sodium malonate (dibasic monohydrate) and equilibrated against a reservoir containing 100 micro-l. |