7BLT
Notum_Maybridge_4
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-07-29 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.97627 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 59.700, 72.320, 78.190 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.160 - 1.200 |
R-factor | 0.1998 |
Rwork | 0.199 |
R-free | 0.21100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4uz1 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | MOLREP |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.040 | 1.220 |
High resolution limit [Å] | 1.200 | 1.200 |
Number of reflections | 99343 | 284 |
<I/σ(I)> | 14.87 | |
Completeness [%] | 93.5 | |
Redundancy | 11.7 | |
CC(1/2) | 0.999 | 0.508 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 296 | 1.5 M Ammonium Sulphate 0.1 M Sodium Citrate, pH4.2 |