7BLK

Structure of CBM BT3015C from Bacteroides thetaiotaomicron

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-02
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9763
Spacegroup name	P 1 21 1
Unit cell lengths	36.682, 34.291, 47.587
Unit cell angles	90.00, 98.92, 90.00

Refinement procedure

Resolution	36.240 - 1.060
R-factor	0.1685
Rwork	0.168
R-free	0.17480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7blg
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.240	1.080
High resolution limit [Å]	1.060	1.060
Number of reflections	84450	2611
<I/σ(I)>	10.5
Completeness [%]	98.6
Redundancy	3
CC(1/2)	0.990	0.920

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	8 mg/ml 30% w/v PEG 2000 monomethyl ether and 0.1 M potassium thiocyanate