7BK8
X-ray crystal structure of Pseudomonas aeruginosa MagC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE W01B-MX2 |
| Synchrotron site | LNLS |
| Beamline | W01B-MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-06-29 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.458680 |
| Spacegroup name | H 3 |
| Unit cell lengths | 110.737, 110.737, 41.110 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.785 - 1.740 |
| Rwork | 0.193 |
| R-free | 0.22190 |
| Structure solution method | AB INITIO PHASING |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.267 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | Arcimboldo |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.790 | 1.850 |
| High resolution limit [Å] | 1.740 | 1.740 |
| Number of reflections | 18977 | 3021 |
| <I/σ(I)> | 26.91 | 3 |
| Completeness [%] | 98.4 | 93.7 |
| Redundancy | 6.6 | 4.9 |
| CC(1/2) | 1.000 | 0.889 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 293 | 30% w/v PEG6000, 100 mM Citrate pH 6.5 |
