7BJP
The cryo-EM structure of vesivirus 2117, an adventitious agent and possible cause of haemorrhagic gastroenteritis in dogs.
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | JEOL CRYO ARM 300 |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Detector | DIRECT ELECTRON DE-64 (8k x 8k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.65 |
| Number of Particle | 65071 |
| Software | RELION,PHENIX,REFMAC,JADAS,Gctf,Coot |
| Symmetry | I |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
