7BJN

Crystal structure of atypical Tm1 (Tm1-I/C), residues 270-334

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-08-31
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0723
Spacegroup name	H 3
Unit cell lengths	127.880, 127.880, 34.170
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	63.940 - 2.300
R-factor	0.2357
Rwork	0.234
R-free	0.26950
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	poly Ala helix
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	AMPLE
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	63.940	63.940	2.360
High resolution limit [Å]	2.300	10.280	2.300
Rmerge	0.093	0.033	0.984
Rmeas	0.103	0.037	1.097
Total number of observations	48426
Number of reflections	9264	106	664
<I/σ(I)>	10.45	37.35	1.33
Completeness [%]	99.8	99.1	98.2
Redundancy	5.227	5.5	5.066
CC(1/2)	0.998	0.999	0.559

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	293	0.1 M NH4Ac pH 4.6, 15% (w/v) PEG 4000