7BJI

Crystal structure of the Danio rerio centrosomal protein Cep135 coiled-coil fragment 64-190

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-03
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9763
Spacegroup name	C 2 2 2
Unit cell lengths	103.290, 188.220, 89.970
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	63.820 - 2.580
R-factor	0.234
Rwork	0.233
R-free	0.24900
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.000
Data reduction software	XDS
Data scaling software	pointless
Phasing software	SHELXDE
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	89.970	2.620
High resolution limit [Å]	2.580	2.580
Rmeas	0.091	1.900
Number of reflections	28078	1392
<I/σ(I)>	12.5	1.1
Completeness [%]	99.9	100
Redundancy	6.6	6.9
CC(1/2)	1.000	0.500

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4	277	15 mg/ml DrCep135 64-190 in 20 mM HEPES pH 7.5, 150 mM NaCl, 0.5 mM TCEP; 0.2 M ammonium acetate, 0.1 M sodium acetate, 15% w/v PEG 4000, pH 4 mother liquor; 1:2 protein / mother liquor ratio, 300nl drops