7BJG

Crystal structure of atypical Tm1 (Tm1-I/C), residues 262-363

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-08-31
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0723
Spacegroup name	P 6 2 2
Unit cell lengths	107.030, 107.030, 52.670
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	53.520 - 2.450
R-factor	0.2543
Rwork	0.252
R-free	0.29410
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	poly A helix
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	AMPLE
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	53.520	53.520	2.510
High resolution limit [Å]	2.450	10.940	2.450
Rmerge	0.091	0.063	1.067
Rmeas	0.094	0.066	1.097
Total number of observations	129216
Number of reflections	6906	106	493
<I/σ(I)>	17.55	35.03	2.21
Completeness [%]	99.1	97.2	96.9
Redundancy	18.711	13.689	18.124
CC(1/2)	0.999	0.999	0.942

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	0.1 M Tris-HCl pH8, 40% MPD