7BIP
Crystal structure of monooxygenase RslO1 from Streptomyces bottropensis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-08-27 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 50.912, 81.962, 160.624 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.530 - 1.600 |
R-factor | 0.1659 |
Rwork | 0.164 |
R-free | 0.19390 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2i7g |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MrBUMP |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.530 | 1.630 |
High resolution limit [Å] | 1.600 | 1.600 |
Number of reflections | 89593 | 4319 |
<I/σ(I)> | 13.3 | |
Completeness [%] | 100.0 | |
Redundancy | 25.8 | |
CC(1/2) | 0.999 | 0.542 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M calcium chloride, 0.1 M HEPES pH 7.0 and 33% polyethylene glycol |