7BIO

Crystal structure of monooxygenase RslO4 from Streptomyces bottropensis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-30
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1
Spacegroup name	P 32 2 1
Unit cell lengths	83.681, 83.681, 82.346
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.840 - 1.795
R-factor	0.1839
Rwork	0.183
R-free	0.20420
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3kng
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	72.470	1.912
High resolution limit [Å]	1.795	1.795
Number of reflections	25998	1300
<I/σ(I)>	25.7
Completeness [%]	94.4
Redundancy	19.7
CC(1/2)	1.000	0.638

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	6% ethanol, 0.1 M ammonium acetate (pH 5.5) and 1.5 M phosphate (pH 7.0)