7BIH
Crystal structure of RecJ-Cdc45 from Methanothermobacter Thermoautotrophicus in the closed state.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-06-21 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.149, 101.629, 91.078 |
| Unit cell angles | 90.00, 96.08, 90.00 |
Refinement procedure
| Resolution | 41.300 - 3.000 |
| R-factor | 0.2143 |
| Rwork | 0.210 |
| R-free | 0.28260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6tvv |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.492 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.500 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 19632 | 2851 |
| <I/σ(I)> | 4.5 | |
| Completeness [%] | 99.8 | |
| Redundancy | 8.7 | |
| CC(1/2) | 0.990 | 1.000 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1M Tris/HCl pH 8.5, 27% w/v PEG 4000, 0.2M MgCl2*6H20, 1mM MnCl2 |
