7BIF
Crystal structure of v22WRAP-T, a 7-bladed designer protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-07-30 |
Detector | DECTRIS EIGER2 XE 16M |
Wavelength(s) | 0.9763 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 63.853, 72.886, 93.545 |
Unit cell angles | 90.00, 96.00, 90.00 |
Refinement procedure
Resolution | 50.070 - 1.400 |
R-factor | 0.1572 |
Rwork | 0.156 |
R-free | 0.18130 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2ymu |
RMSD bond length | 0.006 |
RMSD bond angle | 1.010 |
Data reduction software | DIALS (3.0.4) |
Data scaling software | Aimless (CCP4Interface 6.5.000) |
Phasing software | PHASER (CCP4Interface 6.5.000) |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 57.370 | 1.420 |
High resolution limit [Å] | 1.400 | 1.400 |
Rmerge | 0.039 | 0.191 |
Rmeas | 0.042 | 0.214 |
Rpim | 0.016 | 0.094 |
Number of reflections | 166377 | 7202 |
<I/σ(I)> | 25.5 | 6.2 |
Completeness [%] | 99.2 | 86.5 |
Redundancy | 6.5 | 4.9 |
CC(1/2) | 1.000 | 0.969 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 0.2M Sodium thiocyanate pH 6.9, 20%(w/v)PEG 3350 |