7BHL

Crystal structure of hen egg white lysozyme using drop-on-drop SFX method - 0.2 s mixing with N-acetyl-D-glucosamine.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	FREE ELECTRON LASER
Source details	SACLA BEAMLINE BL2
Synchrotron site	SACLA
Beamline	BL2
Temperature [K]	308
Detector technology	CCD
Collection date	2019-07-11
Detector	RAYONIX MX300-HS
Wavelength(s)	1.24
Spacegroup name	P 43 21 2
Unit cell lengths	78.804, 78.804, 38.002
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.230 - 1.450
R-factor	0.1978
Rwork	0.197
R-free	0.21080
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4eta
RMSD bond length	0.005
RMSD bond angle	0.757
Phasing software	PHASER
Refinement software	PHENIX (1.18.1_3865)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.720	1.475
High resolution limit [Å]	1.450	1.450
Number of reflections	21782	1056
<I/σ(I)>	55.75	0.497
Completeness [%]	100.0	100
Redundancy	52.84
CC(1/2)	0.940	0.030

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE		295	1 M citric acid, 20% (w/v) NaCl, 5% (w/v) PEG 6000, pH 3.0