7BGS
Archeal holliday junction resolvase from Thermus thermophilus phage 15-6
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-04-26 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 97.580, 103.710, 84.850 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.150 - 2.500 |
R-factor | 0.2372 |
Rwork | 0.236 |
R-free | 0.25810 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 0.940 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | CRANK2 |
Refinement software | BUSTER (2.11.8) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.200 | 2.600 |
High resolution limit [Å] | 2.500 | 2.500 |
Number of reflections | 253474 | 17436 |
<I/σ(I)> | 18.2 | |
Completeness [%] | 99.9 | |
Redundancy | 16.7 | |
CC(1/2) | 0.999 | 0.631 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 1.8 M ammonium sulphate and 0.1 M sodium acetate pH 4.2 |