7BGF

CRYSTAL STRUCTURE OF THE N-TERMINAL DIMERIC COILED COIL OF THE HUMAN CTIP PROTEIN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-08-15
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.976251
Spacegroup name	P 32
Unit cell lengths	86.598, 86.598, 42.601
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.500 - 2.802
Rwork	0.224
R-free	0.25280
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Homology models
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.500	2.902
High resolution limit [Å]	2.802	2.802
Rmerge	0.055	0.758
Rmeas	0.068	0.936
Rpim	0.039	0.543
Number of reflections	8624	878
<I/σ(I)>	12.18	1.29
Completeness [%]	98.0	99.1
Redundancy	2.8	2.8
CC(1/2)	0.999	0.601

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	Condition F6 of the Morpheus crystallisation screen MD-47 (Molecular Dimensions).