7BG1
Structure of anti-FLAG M2 Fab domain remodeled based on proteomic sequencing
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-06-17 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 87.360, 133.760, 41.480 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.520 - 1.860 |
| R-factor | 0.2192 |
| Rwork | 0.217 |
| R-free | 0.25520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2g60 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.727 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.033 | 0.249 |
| Number of reflections | 46087 | 2659 |
| <I/σ(I)> | 19 | |
| Completeness [%] | 92.3 | 58.9 |
| Redundancy | 4.2 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 15% PEG 4000, 0.1M ammonium sulfate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
