7BFL
X-ray structure of SS-RNase-2 des116-120
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2020-07-06 |
Detector | RIGAKU SATURN 944 |
Wavelength(s) | 1.5418 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 110.070, 93.070, 66.780 |
Unit cell angles | 90.00, 117.19, 90.00 |
Refinement procedure
Resolution | 67.460 - 2.880 |
R-factor | 0.2613 |
Rwork | 0.259 |
R-free | 0.30760 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7bfk |
RMSD bond length | 0.003 |
RMSD bond angle | 1.143 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 70.000 | 3.060 |
High resolution limit [Å] | 2.880 | 2.880 |
Number of reflections | 13043 | 1832 |
<I/σ(I)> | 3.4 | 1.2 |
Completeness [%] | 96.2 | |
Redundancy | 3.4 | |
CC(1/2) | 0.889 | 0.411 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 25% w/v PEG 3350, 0.2 M sodium chloride and 0.1 M Bis-Tris pH 6.5 |