7BFK
X-ray structure of SS-RNase-2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-12-02 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 28.968, 70.747, 53.545 |
| Unit cell angles | 90.00, 90.93, 90.00 |
Refinement procedure
| Resolution | 26.820 - 1.890 |
| R-factor | 0.1859 |
| Rwork | 0.184 |
| R-free | 0.21880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vq8 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.449 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.960 |
| High resolution limit [Å] | 1.890 | 1.890 |
| Rmerge | 0.072 | 0.310 |
| Number of reflections | 17350 | 1684 |
| <I/σ(I)> | 15.2 | 2.4 |
| Completeness [%] | 99.4 | 96.3 |
| Redundancy | 3 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | 32% w/v PEG 4000, 0.2 M sodium acetate trihydrate, 0.1 M Tris-HCl pH 7.4 and 1% v/v acetonitrile |
