7BFG

Circular permutant of ribosomal protein S6, P54-55 truncated, V37A mutant.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-16
Detector	PSI PILATUS 6M
Wavelength(s)	0.97625
Spacegroup name	P 21 21 21
Unit cell lengths	62.315, 75.933, 223.737
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	111.870 - 2.010
R-factor	0.249
Rwork	0.247
R-free	0.27930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ris
RMSD bond length	0.007
RMSD bond angle	1.365
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	111.870	111.868	2.229
High resolution limit [Å]	2.010	6.576	2.029
Rmerge	0.197	0.084	1.247
Rmeas	0.215	0.092	1.351
Rpim	0.085	0.039	0.513
Number of reflections	45056	2253	2253
<I/σ(I)>	6.7
Completeness [%]	90.5	99.7	72.6
Redundancy	6.5	5.5	6.8
CC(1/2)	0.996	0.999	0.701

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	293	0.1 M citrate 20% w/v PEG 6000