7BFF
Circular permutant of ribosomal protein S6, P54-55 truncated, I25A mutant.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P13 (MX1) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-09-16 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.97625 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 38.691, 68.070, 222.357 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.190 - 1.660 |
R-factor | 0.2112 |
Rwork | 0.209 |
R-free | 0.25260 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ris |
RMSD bond length | 0.015 |
RMSD bond angle | 1.980 |
Data reduction software | XDS |
Data scaling software | Aimless (0.6.2) |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 68.070 | 68.070 | 1.690 |
High resolution limit [Å] | 1.660 | 9.080 | 1.660 |
Rmerge | 0.072 | 0.057 | 0.856 |
Rmeas | 0.079 | 0.063 | 0.929 |
Rpim | 0.032 | 0.027 | 0.358 |
Total number of observations | 370499 | 2749 | 23417 |
Number of reflections | 59779 | 531 | 3515 |
<I/σ(I)> | 12.9 | 28.9 | 2.3 |
Completeness [%] | 83.8 | 97.7 | 99.9 |
Redundancy | 6.2 | 5.2 | 6.7 |
CC(1/2) | 0.994 | 0.992 | 0.873 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M sodium thiocyanate 20% w/v PEG 3350 |