7BFF
Circular permutant of ribosomal protein S6, P54-55 truncated, I25A mutant.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
| Synchrotron site | PETRA III, EMBL c/o DESY |
| Beamline | P13 (MX1) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-09-16 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 38.691, 68.070, 222.357 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.190 - 1.660 |
| R-factor | 0.2112 |
| Rwork | 0.209 |
| R-free | 0.25260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ris |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.980 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.6.2) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 68.070 | 68.070 | 1.690 |
| High resolution limit [Å] | 1.660 | 9.080 | 1.660 |
| Rmerge | 0.072 | 0.057 | 0.856 |
| Rmeas | 0.079 | 0.063 | 0.929 |
| Rpim | 0.032 | 0.027 | 0.358 |
| Total number of observations | 370499 | 2749 | 23417 |
| Number of reflections | 59779 | 531 | 3515 |
| <I/σ(I)> | 12.9 | 28.9 | 2.3 |
| Completeness [%] | 83.8 | 97.7 | 99.9 |
| Redundancy | 6.2 | 5.2 | 6.7 |
| CC(1/2) | 0.994 | 0.992 | 0.873 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M sodium thiocyanate 20% w/v PEG 3350 |
