7BFE
Circular permutant of ribosomal protein S6, P54-55 truncated, L21A mutant.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
| Synchrotron site | PETRA III, EMBL c/o DESY |
| Beamline | P13 (MX1) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-09-16 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 39.000, 67.490, 221.950 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 64.650 - 1.950 |
| R-factor | 0.235 |
| Rwork | 0.233 |
| R-free | 0.28040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ris |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.945 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.6.2) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 64.900 | 64.570 | 2.000 |
| High resolution limit [Å] | 1.950 | 8.940 | 1.950 |
| Rmerge | 0.066 | 0.036 | 0.404 |
| Rmeas | 0.079 | 0.045 | 0.489 |
| Rpim | 0.043 | 0.027 | 0.271 |
| Total number of observations | 1317 | 8756 | |
| Number of reflections | 42847 | 508 | 2953 |
| <I/σ(I)> | 8 | 19.3 | 2.2 |
| Completeness [%] | 98.0 | 92.7 | 98.8 |
| Redundancy | 2.9 | 2.6 | 3 |
| CC(1/2) | 0.989 | 0.986 | 0.887 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.2 M sodium iodide 0.1 M Bis-Tris propane 20% w/v PEG 3350 |
