7BE2
X-ray structure of Hen Egg White Lysozyme with dirhodium tetraacetate (6)
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 11.2C |
| Synchrotron site | ELETTRA |
| Beamline | 11.2C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-11-19 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.5406 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 78.930, 78.930, 37.120 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 55.810 - 1.650 |
| Rwork | 0.182 |
| R-free | 0.23410 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 193l |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.546 |
| Data reduction software | MOSFLM |
| Data scaling software | MOSFLM |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.812 | 1.800 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.120 | 3.230 |
| Rpim | 0.025 | 0.680 |
| Number of reflections | 11800 | 591 |
| <I/σ(I)> | 22.5 | 1.3 |
| Completeness [%] | 94.0 | 52.6 |
| Redundancy | 24.5 | 22.4 |
| CC(1/2) | 1.000 | 0.510 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.01 M HEPES, 2.00 M sodium formate |
