7BE1
X-ray structure of Hen Egg White Lysozyme with dirhodium tetraacetate (3)
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 11.2C |
| Synchrotron site | ELETTRA |
| Beamline | 11.2C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-06-02 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.5406 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 78.360, 78.360, 37.610 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 55.409 - 1.400 |
| Rwork | 0.190 |
| R-free | 0.20890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 193l |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.866 |
| Data reduction software | MOSFLM |
| Data scaling software | MOSFLM |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 78.360 | 1.470 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.099 | 2.000 |
| Rpim | 0.023 | 0.519 |
| Number of reflections | 23665 | 3274 |
| <I/σ(I)> | 10.2 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 19.6 | 16.6 |
| CC(1/2) | 0.999 | 0.701 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 0.1 M sodium acetate, 20% ethylene glicol, 0.6 M sodium nitrate |
