7BDU
Crystal structure of a Hsp47-collagen peptide complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-10-24 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1.0 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 91.821, 129.800, 173.372 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.910 - 2.490 |
R-factor | 0.2201 |
Rwork | 0.217 |
R-free | 0.26700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4au2 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.910 | 2.590 |
High resolution limit [Å] | 2.490 | 2.490 |
Rmerge | 0.120 | 0.800 |
Rmeas | 0.125 | |
Number of reflections | 68330 | 3406 |
<I/σ(I)> | 10.12 | |
Completeness [%] | 99.0 | |
Redundancy | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 0.1 M HEPES, 18-26% PEG 3350, 1-6% Tacsimate pH 7.0 |