7BD7
X-ray structure of Arsenoplatin-1-encapsulated horse spleen ferritin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-3 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-3 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-07-10 |
| Detector | DECTRIS EIGER2 X 4M |
| Wavelength(s) | 0.9677 |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 180.910, 180.910, 180.910 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.540 - 1.500 |
| R-factor | 0.1673 |
| Rwork | 0.166 |
| R-free | 0.18510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5erk |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.819 |
| Data reduction software | iMOSFLM |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.538 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.113 | 0.840 |
| Rmeas | 0.120 | 0.891 |
| Rpim | 0.040 | 0.404 |
| Number of reflections | 40897 | 2028 |
| <I/σ(I)> | 12.4 | 2.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 8.7 | 9.1 |
| CC(1/2) | 0.999 | 0.563 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 293 | 0.6 M ammomium sulphate 0.1 M Tris HCl buffer pH 7.2 60 mM cadmium sulphate |
