7BCU

The adduct of NAMI-A with Hen Egg White Lysozyme at 1.5 hours.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-08-11
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.9763
Spacegroup name	P 43 21 2
Unit cell lengths	77.419, 77.419, 37.643
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.020 - 0.980
R-factor	0.1277
Rwork	0.127
R-free	0.14430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	193l
RMSD bond length	0.005
RMSD bond angle	0.861
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.853	1.015
High resolution limit [Å]	0.980	0.980
Rmerge	0.068	0.179
Number of reflections	65524	6106
<I/σ(I)>	27.98	3.69
Completeness [%]	99.3
Redundancy	23
CC(1/2)	0.999	0.923

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	289	NACL 0.7-1.5 M, 0.1 M NA ACETATE, PH 4.6, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 289K