7BCN
Crystal structure of the sugar acid binding protein DctPAm from Advenella mimigardefordensis strain DPN7T in complex with Xylonic acid
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-12-12 |
Detector | DECTRIS EIGER R 4M |
Wavelength(s) | 0.967 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 58.110, 62.349, 97.645 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.060 - 1.700 |
R-factor | 0.1731 |
Rwork | 0.172 |
R-free | 0.20250 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7bbr |
RMSD bond length | 0.013 |
RMSD bond angle | 1.250 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.060 | 1.760 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.057 | 0.420 |
Number of reflections | 159256 | 16753 |
<I/σ(I)> | 15.12 | |
Completeness [%] | 96.5 | 86 |
Redundancy | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 298 | Peg4000 pH 7.2 |