7BCA
Crystal structure of the HTH DNA binding protein ArdK from R388 plasmid bound to a direct-repeat DNA element
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 105 |
Detector technology | CCD |
Collection date | 2009-05-22 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9790 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 88.120, 39.060, 120.040 |
Unit cell angles | 90.00, 101.65, 90.00 |
Refinement procedure
Resolution | 58.780 - 2.800 |
R-factor | 0.2345 |
Rwork | 0.232 |
R-free | 0.29260 |
Structure solution method | SAD |
RMSD bond length | 0.004 |
RMSD bond angle | 0.720 |
Data reduction software | MOSFLM (7.2.2) |
Data scaling software | SCALA (CCP4 7.0.078) |
Phasing software | PHENIX (1.17.1_3660) |
Refinement software | PHENIX (1.20_4459) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 58.780 | 2.950 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.051 | 0.335 |
Rmeas | 0.071 | 0.462 |
Number of reflections | 10025 | 1437 |
<I/σ(I)> | 10.4 | 2.7 |
Completeness [%] | 98.9 | |
Redundancy | 3.4 | |
CC(1/2) | 0.997 | 0.901 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295 | 20% PEG 4,000, 10% 2-propanol, 0.1 M Tris-HCl [pH 7.5] |