7BBQ

Crystal structure of the HTH DNA binding protein ArdK from R388 plasmid. Apo form.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM16
Synchrotron site	ESRF
Beamline	BM16
Temperature [K]	110
Detector technology	CCD
Collection date	2007-04-19
Detector	ADSC QUANTUM 210
Wavelength(s)	0.9794
Spacegroup name	P 41 21 2
Unit cell lengths	43.100, 43.100, 190.890
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.100 - 3.000
R-factor	0.2478
Rwork	0.246
R-free	0.27080
Structure solution method	SAD
RMSD bond length	0.013
RMSD bond angle	1.442
Data reduction software	MOSFLM (7.2.2)
Data scaling software	SCALA (CCP4 7.0.078)
Phasing software	PHENIX (1.17.1_3660)
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	63.630	3.160
High resolution limit [Å]	3.000	3.000
Rmerge	0.097	0.371
Rmeas	0.112	0.428
Number of reflections	4103	565
<I/σ(I)>	11.3	3.8
Completeness [%]	99.8
Redundancy	6.6
CC(1/2)	0.992	0.963

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	20 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA