7BBP
Crystal Structure of the second bromodomain of Pleckstrin homology domain interacting protein (PHIP) in complex with H4K5acK8ac
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-06-20 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.9282 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.210, 58.310, 77.120 |
| Unit cell angles | 90.00, 99.08, 90.00 |
Refinement procedure
| Resolution | 55.390 - 1.990 |
| Rwork | 0.205 |
| R-free | 0.24050 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3mb3 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.456 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.390 | 2.040 |
| High resolution limit [Å] | 1.990 | 1.990 |
| Number of reflections | 41404 | 2999 |
| <I/σ(I)> | 11.3 | |
| Completeness [%] | 99.0 | |
| Redundancy | 3.4 | |
| CC(1/2) | 0.998 | 0.653 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | 30% 2-propanol -- 0.2M ammonium acetate -- 0.1M tris pH 8.5 |
