7BBL

Structure of human Gemin6/Gemin7/Gemin8 trimeric complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	77
Detector technology	PIXEL
Collection date	2016-11-25
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0
Spacegroup name	P 2 21 21
Unit cell lengths	59.880, 80.597, 82.666
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.494 - 1.521
R-factor	0.208
Rwork	0.206
R-free	0.25060
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1y96
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.15.1_3469)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.494	1.576
High resolution limit [Å]	1.521	1.521
Rmerge	0.067	0.793
Rmeas	0.076	0.960
Rpim	0.035	0.523
Number of reflections	58263	4340
<I/σ(I)>	10.83	1.03
Completeness [%]	93.5	70.86
Redundancy	3.8	2.3
CC(1/2)	0.999	0.197

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	100 mM 2-(N-morpholino) ethanesulfonic acid, 200 mM NaCl, 30 % Jeffamine ED2003